IJMCM Vol3, No3, 166-175 Dr. Bouzari [ARaRVTSCP] 93-4-31
نویسندگان
چکیده
Neisseria meningitidis is a major causative agent of bacterial septicemia and meningitis in humans. Currently, there are no vaccines to prevent disease caused by strains of N. meningitidis serogroup B. The Class 1 Outer Membrane Protein (OMP) has been named porA which is a cation selective transmembrane protein of 45 KDa that forms trimeric pore in the meningococcal outer membrane. PorA from serogroup B N. meningitidis was cloned into prokaryotic expression vector pBAD-gIIIA. Recombinant protein was expressed with arabinose and affinity purified by Ni-NTA agarose, SDS-PAGE and western blotting were performed for protein determination and verification. BALB/c mice were immunized subcutaneously with purified rPorA together with alum adjuvant. Serum antibody responses to serogroups B N. meningitidis were determined by ELISA. Serum IgG response significantly increased in the group immunized with rPorA together with alum adjuvant in comparison with control groups. These results suggest that rPorA can be a potential vaccine candidate for serogroup B N. meningitidis.
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1. Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran. 2. Department of Anesthesiology, Roohani Hospital, Babol University of Medical Sciences, Babol, Iran. 3. Department of Pharmacology & Physiology, School of Medicine, Babol University of Medical Sciences, Babol, Iran. 4. Student Research Committee, Babol university of medical Sciences, Babol, Iran.
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1. Dental Materials Research Center, School of Dentistry, Babol University of Medical Sciences, Babol, Iran. 2. Department of Bacteriology and Virology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran. 3. Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran. 4. Department of Pharmacology & Physiology, School of Medicine...
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